Paperbase

LM14 defined medium enables continuous growth of Trypanosoma cruzi

De Paula Lima, Carla V. Batista, Michel Kugeratski, Fernanda G. Vincent, Isabel M. Soares, Maurilio J. Probst, Christian M. Krieger, Marco A. Marchini, Fabricio K.

BMC Microbiology, 2014

https://doi.org/10.1186/s12866-014-0238-y

Fernanda Kugeratski

Kugeratski Fernanda

ORCID: 0000-0001-9731-1275

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Maurilio Soares

Soares Maurilio

ORCID: 0000-0001-9701-836X

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Christian Probst

Probst Christian / Probst, C.M.

ORCID: 0000-0001-9993-6375

Email: cmacprobst@gmail.com

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Vincent Isabel

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Fabricio Marchini

Marchini Fabricio / Marchini FK

ORCID: 0000-0001-5695-2746

Email: marchinifk@fiocruz.br

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Synthesis and antibacterial activity of alkylated diamines and amphiphilic amides of quinic acid derivatives 10.1111/cbdd.12498

pTcGW plasmid vectors 1.1 version: A versatile tool for Trypanosoma cruzi gene characterisation 10.1590/0074-02760150074

The translation initiation complex eIF3 in trypanosomatids and other pathogenic excavates--identification of conserved and divergent features based on orthologue analysis. 10.1186/1471-2164-15-1175

Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts 10.1089/scd.2013.0496

LM14 defined medium enables continuous growth of Trypanosoma cruzi 10.1186/s12866-014-0238-y

The mRNAs associated to a zinc finger protein from Trypanosoma cruzi shift during stress conditions 10.4161/rna.29622

Towards the phosphoproteome of trypanosomatids 10.1007/978-94-007-7305-9_15

A high-throughput cloning system for reverse genetics in Trypanosoma cruzi 10.1186/1471-2180-10-259

Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database 10.1016/j.gene.2009.08.007

Profiling the Trypanosoma cruzi Phosphoproteome 10.1371/journal.pone.0025381

Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi 10.1111/j.1742-4658.2010.07747.x

Quantitative proteomics of Trypanosoma cruzi during metacyclogenesis 10.1002/pmic.201200078

Trypanosoma cruzi: a stage-specific calpain-like protein is induced after various kinds of stress 10.1590/s0074-02762008000600015

Characterising ISWI chromatin remodeler in Trypanosoma cruzi 10.1590/0074-02760190457

Label-free aptasensor for p24-HIV protein detection based on graphene quantum dots as an electrochemical signal amplifier 10.1016/j.aca.2021.338548

Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi 10.1016/j.jprot.2020.103902

Integrated analysis of label-free quantitative proteomics and bioinformatics reveal insights into signaling pathways in male breast cancer 10.1590/1678-4685-GMB-2019-0410

Phylogenetic characterization of arboviruses in patients suffering from acute fever in Rondônia, Brazil 10.3390/v12080889

Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family 10.1371/journal.pone.0060209

Genome-Wide Proteomics and Phosphoproteomics Analysis of Trypanosoma cruzi During Differentiation 10.1007/978-1-0716-0294-2_11

Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: Investigating dengue cross-reaction 10.1101/2020.10.09.20210039

Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral load 10.1038/s41598-021-88506-y

Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion 10.1007/s42770-021-00556-6

Diagnostics and the neglected tropical diseases roadmap: Setting the agenda for 2030 10.1093/trstmh/traa118

Analysis of the Biotechnological Potential of a Lentinus crinitus Isolate in the Light of Its Secretome 10.1021/acs.jproteome.6b00636

Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion 10.1101/2021.02.19.21252100

A low-cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters 10.1007/s10544-021-00563-2

Marco Aurelio Krieger

Krieger Marco Aurelio

ORCID: 0000-0003-0030-3952

The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis 10.1590/S0074-02762012000600014

Advances to Bordetella pertussis diagnosis: Adapting a real time PCR to a lab-on-chip platform 10.1109/SBMicro.2016.7731331

Involvement of STI1 protein in the differentiation process of Trypanosoma cruzi 10.1016/j.parint.2017.10.009

Electrospinning induced surface activation (EISA) of highly porous PMMA microfiber mats for HIV diagnosis 10.1039/c6tb01435f

Use of recombinant antigens for the accurate immunodiagnosis of Chagas' disease 10.4269/ajtmh.1992.46.427

Study of VIPER and TATE in kinetoplastids and the evolution of tyrosine recombinase retrotransposons 10.1186/s13100-019-0175-2

Dengue virus infections: Comparison of methods for diagnosing the acute disease 10.1016/j.jcv.2004.08.008

Expression and polymorphism of a Trypanosoma cruzi gene encoding a cytoplasmic repetitive antigen 10.1016/0014-4894(90)90106-M

DNA sequencing of 13 cytokine gene fragments of Aotus infulatus and Saimiri sciureus, two non-human primate models for malaria. 10.1016/j.cyto.2010.09.004

Development of Hepatitis C virus genotyping by real-time PCR based on the NS5B region 10.1371/journal.pone.0010150

Demonstration of an On-Chip Real-Time PCR for the Detection of Trypanosoma Cruzi 10.1007/978-3-319-00846-2_216

Trypanosoma cruzi: A gene family encoding chitin-binding-like proteins is posttranscriptionally regulated during metacyclogenesis 10.1006/expr.2001.4628

Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C 10.1186/1743-422X-8-459

Inhibition of proteasome activity blocks Trypanosoma cruzi growth and metacyclogenesis 10.1007/s00436-008-1081-6

Use of the EIE-Recombinant-Chagas-Biomanguinhos kit to monitor cure of human Chagas' disease 10.1002/jcla.10028

Brazilian response to global end TB strategy: The national tuberculosis research agenda 10.1590/0037-8682-0330-2015

Ultra-sensitive detection of mycobacterium leprae: DNA extraction and PCR assays 10.1371/journal.pntd.0008325

External Control Viral-Like Particle Construction for Detection of Emergent Arboviruses by Real-Time Reverse-Transcription PCR 10.1155/2019/2560401

Recombinant antigen production for assays of intradermoreaction for diagnosis and surveillance of tuberculosis 10.1016/j.jbiotec.2011.07.015

Development of a magnetic separation method to capture sepsis associated bacteria in blood 10.1016/j.mimet.2016.07.012

Post-translational Modifications of Trypanosoma cruzi Canonical and Variant Histones 10.1021/acs.jproteome.6b00655

Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices 10.1016/j.fawpar.2021.e00111

Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis 10.1038/s41598-017-10292-3

Knocking Down TcNTPDase-1 Gene Reduces in vitro Infectivity of Trypanosoma cruzi 10.3389/fmicb.2020.00434

Crystal structure of chagasin, the endogenous cysteine-protease inhibitor from Trypanosoma cruzi 10.1016/j.jsb.2006.07.017

A high-throughput cloning system for reverse genetics in Trypanosoma cruzi 10.1186/1471-2180-10-259

Chagas' disease diagnosis: evaluation of several tests in blood bank screening 10.1046/j.1537-2995.1993.331094054620.x

Hepatitis C virus quantification in serum and saliva of HCV-infected patients 10.1590/S0074-02762012000500016

Surveillance of donated blood during the 2016 arbovirus outbreak in Brazil 10.1002/jmv.25193

The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism 10.1016/j.jprot.2016.12.011

Molecular characterization of type II topoisomerase in the endosymbiont-bearing Trypanosomatid Blastocrithidia culicis 10.1111/j.1574-6968.2006.00164.x

Gene expression profiling of macrophages following mice treatment with an immunomodulator medication 10.1002/jcb.21713

Developing self-generated calibration curves using a capillary-driven wax-polyester lab on a chip device and thermal gates 10.1016/j.microc.2019.01.062

Viability study of a multiplex diagnostic platform for Chagas disease 10.1590/S0074-02762009000900019

Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain 10.1107/S1744309109017734

Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics 10.1590/0074-02760160380

Sera of chagasic patients react with antigens from the tomato parasite Phytomonas serpens 10.4067/S0716-97602010000200011

Trypanosoma cruzi: Cloning and characterization of two genes whose expression is up-regulated in metacyclic trypomastigotes 10.1016/j.actatropica.2003.10.018

Development of a New Lateral Flow Assay Based on IBMP-8.1 and IBMP-8.4 Chimeric Antigens to Diagnose Chagas Disease 10.1155/2020/1803515

In vitro formation of capillary tubules from human umbilical cord blood cells with perspectives for therapeutic application,Formação in vitro de túbulos capilares a partir de células de sangue de cordão umbilical humano com perspectivas para aplicação terapêutica 10.1590/s0102-76382008000400003

Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix 10.1016/j.ijid.2021.01.001

Profiling the Trypanosoma cruzi phosphoproteome 10.1371/journal.pone.0025381

Evidence of P-body-like structures in Trypanosoma cruzi 10.1016/j.bbrc.2007.03.104

Evidence for an ATP-sensitive K⁺ channel in mitoplasts isolated from Trypanosoma cruzi and Crithidia fasciculata 10.1016/j.ijpara.2009.01.002

Detection of IgG3 antibodies specific to the human immunodeficiency virus type 1 (HIV-1) p24 protein as marker for recently acquired infection 10.1017/S0950268818001218

Epidemiological profile of zika, dengue and chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil 10.1590/s1678-9946201961040

Proof of Concept for a Portable Platform for Molecular Diagnosis of Tropical Diseases: On-Chip Ready-to-Use Real-Time Quantitative PCR for Detection of Trypanosoma cruzi or Plasmodium spp. 10.1016/j.jmoldx.2019.04.008

Enhanced target-specific signal detection using an Escherichia coli lysate in multiplex microbead immunoassays with E. coli-derived recombinant antigens 10.1016/j.jim.2016.05.002

Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics 10.1155/2017/9306564

The scavenger receptor MARCO is involved in Leishmania major infection by CBA/J macrophages 10.1111/j.1365-3024.2009.01093.x

Cloning and characterization of the metacyclogenin gene, which is specifically expressed during Trypanosoma cruzi metacyclogenesis 10.1016/S0166-6851(01)00346-2

Assessment of clinical and epidemiological characteristics of patients with and without sepsis in intensive care units of a tertiary hospital 10.31744/einstein_journal/2019AO4476

A clinical scoring system to predict long-term arthralgia in chikungunya disease: A cohort study 10.1371/journal.pntd.0008467

Correction: High accuracy of an elisa test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil—A phase II study (PLoS Negl Trop Dis, (2018) 12, 10, 10.1371/journal.pntd.0006871) 10.1371/journal.pntd.0008346

Human cardiac explant-conditioned medium: Soluble factors and cardiomyogenic effect on mesenchymal stem cells 10.1258/ebm.2010.010003

Electrospray induced surface activation of polystyrene microbeads for diagnostic applications 10.1039/c4tb01884b

Proteomic analysis reveals differentially expressed proteins in macrophages infected with Leishmania amazonensis or Leishmania major 10.1016/j.micinf.2013.04.005

Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice 10.1017/S0031182004006043

Use of recombinant antigens for the diagnosis of Chagas disease and blood bank screening. 10.1590/S0074-02761990000400023

Expression profile and subcellular localization of HslV, the proteasome related protease from Trypanosoma cruzi 10.1016/j.exppara.2011.10.011

Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis 10.1371/journal.pone.0234043

Comparison of DNA extraction methods used to detect bacterial and yeast DNA from spiked whole blood by real-time PCR 10.1016/j.mimet.2017.06.020

Differential gene expression in Trypanosoma cruzi populations susceptible and resistant to benznidazole 10.1016/j.actatropica.2008.04.011

Trypanosoma cruzi transcriptome during axenic epimastigote growth curve 10.1590/0074-02760170404

Trypanosoma cruzi: Subtractive hybridization as a molecular strategy to generate new targets to distinguish groups and hybrids 10.1016/j.exppara.2007.04.008

Increased expression of iron-containing superoxide dismutase-A (TcFeSOD-A) enzyme in Trypanosoma cruzi population with in vitro-induced resistance to benznidazole 10.1016/j.actatropica.2006.10.004

Proteomic analysis reveals a predominant NFe2L2 (Nrf2) signature in canonical pathway and upstream regulator analysis of Leishmania-infected macrophages 10.3389/fimmu.2019.01362

Evolutionary analyses of myosin genes in trypanosomatids show a history of expansion, secondary losses and neofunctionalization 10.1038/s41598-017-18865-y

pTcGW plasmid vectors 1.1 version: A versatile tool for Trypanosoma cruzi gene characterisation 10.1590/0074-02760150074

Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes 10.1016/0166-6851(89)90115-1

Mitogen-Activated Protein Kinase Kinase 5 Regulates Proliferation and Biosynthetic Processes in Procyclic Forms of Trypanosoma brucei 10.1021/acs.jproteome.7b00415

Erratum: Trypanosoma cruzi infection induces a global host cell response in cardiomyocytes 10.1128/IAI.05449-11

Towards the phosphoproteome of trypanosomatids 10.1007/978-94-007-7305-9_15

TcZFP1: A CCCH zinc finger protein of Trypanosoma cruzi that binds poly-C oligoribonucleotides in vitro 10.1016/j.bbrc.2004.04.162

Demonstration of a fast and easy sample-to-answer protocol for tuberculosis screening in point-of-care settings: A proof of concept study 10.1371/journal.pone.0242408

Quantitative proteomics of Trypanosoma cruzi during metacyclogenesis 10.1002/pmic.201200078

Optimized broad-range real-time pcr-based method for bacterial screening of platelet concentrates,Otimização de um método baseado em pcr em tempo real para rastreamento de bactérias em concentrados de plaquetas 10.1590/1519-6984.229893

LM14 defined medium enables continuous growth of Trypanosoma cruzi 10.1186/s12866-014-0238-y

Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi 10.1111/j.1742-4658.2010.07747.x

Isolation of an aptamer that binds specifically to E. coli 10.1371/journal.pone.0153637

A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection 10.1186/1471-2180-12-22

Serodiagnosis of Chronic Chagas Infection by Using EIE-Recombinant-Chagas-Biomanguinhos Kit 10.1590/S0074-02762001000400009

Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion. 10.1017/S0031182010001678

Proteomic analysis of metacyclic trypomastigotes undergoing Trypanosoma cruzi metacyclogenesis 10.1002/jms.1267

Are purified or expanded cord blood-derived CD133⁺ cells better at improving cardiac function? 10.1258/ebm.2009.009194

Natural Plasmodium infection in monkeys in the state of Rondônia (Brazilian Western Amazon) 10.1186/1475-2875-12-180

Expression profile of interferon stimulated genes in central nervous system of mice infected with dengue virus Type-1 10.1016/j.virol.2008.04.033

Highly accurate chimeric proteins for the serological diagnosis of chronic chagas disease: A latent class analysis 10.4269/ajtmh.17-0727

A novel hepatitis C virus genotyping method based on liquid microarray 10.1371/journal.pone.0012822

Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi 10.1016/j.ijpara.2008.05.019

High efficiency binding aptamers for a wide range of bacterial sepsis agents 10.4014/jmb.1611.11004

Characterization of the Trypanosoma cruzi ortholog of the SBDS protein reveals an intrinsically disordered extended C-terminal region showing RNA-interacting activity 10.1016/j.biochi.2008.12.001

Expression of WNT10A gene in oral squamous cell carcinoma 10.7727/wimj.2014.347

Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays 10.1128/JCM.00851-17

Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database 10.1016/j.gene.2009.08.007

Trypanosoma cruzii flagellar repetitive antigen expression by recombinant baculovirus: Towards an improved diagnostic reagent for chagas' disease 10.1038/nbt1192-1474

Trypanosoma cruzi: Identification of DNA targets of the nuclear periphery coiled-coil protein TcNUP-1 10.1016/j.exppara.2010.07.009

ProtozoaDB: Dynamic visualization and exploration of protozoan genomes 10.1093/nar/gkm820

Trypanosoma cruzi infection induces a global host cell response in Cardiomyocytes 10.1128/IAI.00643-10

Proteomic analysis reveals the dynamic association of proteins with translated mRNAs in Trypanosoma cruzi 10.1016/j.gene.2009.12.009

Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi 10.1371/journal.pone.0021027

Molecular characterization of the Trypanosoma cruzi specific RNA binding protein TcRBP40 and its associated mRNAs 10.1016/j.bbrc.2012.02.154

High accuracy of an ELISA test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil – A Phase II study 10.1371/journal.pntd.0006871

Development of bioprocess for the production of purified protein derivative with Brazilian strains of Mycobacterium tuberculosis for diagnosis use 10.1016/j.jbiotec.2006.07.007

Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system 10.1016/j.molbiopara.2017.01.005

Genomic and phylogenetic evidence of VIPER retrotransposon domestication in trypanosomatids 10.1590/0074-02760160224

PUMILIO-2 is involved in the positive regulation of cellular proliferation in human adipose-derived stem cells 10.1089/scd.2011.0143

Phylogenetic characterization of arboviruses in patients suffering from acute fever in Rondônia, Brazil 10.3390/v12080889

The mechanism of cysteine detection in biological media by means of vanadium oxide nanoparticles 10.1007/s11051-012-1123-y

Cloning and characterization of a gene encoding a putative protein associated with U3 small nucleolar ribonucleoprotein in Trypanosoma cruzi 10.1016/S0166-6851(02)00247-5

Preliminary multiplex microarray igG immunoassay for the diagnosis of toxoplasmosis and rubella 10.1590/0074-02760160509

Influence of scaffold stability and electrostatics on Top7-based engineered helical HIV-1 epitopes 10.1007/978-3-319-02624-4_9

Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion 10.1016/j.micinf.2009.03.007

Differential Gene Expression during Trypanosoma cruzi Metacyclogenesis 10.1590/S0074-02761999000700021

Trypanosoma cruzi: A stage-specific calpain-like protein is induced after various kinds of stress 10.1590/S0074-02762008000600015

Chronic Chagas disease diagnosis: A comparative performance of commercial enzyme immunoassay tests 10.4269/ajtmh.15-0820

Identification, molecular and functional characterization of calmodulin gene of phytomonas serpens 15T that shares high similarity with its pathogenic counterparts trypanosoma cruzi 10.1007/s10930-011-9322-4

Recently differentiated epimastigotes from Trypanosoma cruzi are infective to the mammalian host 10.1111/mmi.13653

Accuracy of chimeric proteins in the serological diagnosis of chronic chagas disease – a Phase II study 10.1371/journal.pntd.0005433

De novo design of immunoreactive conformation-specific HIV-1 epitopes based on Top7 scaffold 10.1039/c3ra41562g

Trypanosoma cruzi: Molecular characterization of an RNA binding protein differentially expressed in the parasite life cycle 10.1016/j.exppara.2007.03.010

Erratum: Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis (PLoS ONE (2020) 15:6 (e0234043) DOI: 10.1371/journal.pone.0234043) 10.1371/journal.pone.0242386

A ready-to-use duplex qPCR to detect Leishmania infantum DNA in naturally infected dogs 10.1016/j.vetpar.2017.09.009

Swine and poultry pathogens: The complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae 10.1128/JB.187.16.5568-5577.2005

Trypanosoma cruzi Response to Sterol Biosynthesis Inhibitors: Morphophysiological Alterations Leading to Cell Death 10.1371/journal.pone.0055497

Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures 10.1186/s12864-017-4163-y

Representation of differential expression: A new approach to study differential gene expression in trypanosomatids 10.1016/S0169-4758(97)01199-X

Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi 10.1074/jbc.M703097200

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients 10.1128/JCM.02701-20

Characterization of TcSTI-1, a homologue of stress-induced protein-1, in trypanosoma cruzi 10.1590/S0074-02762011000100012

Small-subunit rRNA processome proteins are translationally regulated during differentiation of Trypanosoma cruzi 10.1128/EC.00279-06

Chagas' disease diagnosis: Comparative analysis of recombinant ELISA with conventional ELISA and the haemagglutination test 10.1046/j.1423-0410.2003.00340.x

Characteristics of biofilm formation by Candida tropicalis and antifungal resistance 10.1111/j.1567-1364.2007.00347.x

Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides 10.1186/s12884-018-1774-5

Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection 10.1007/s00436-005-1336-4

Splenic architecture disruption and parasite-induced splenocyte activation and anergy in Plasmodium falciparum-infected Saimiri sciureus monkeys 10.1186/s12936-015-0641-3

Development of a multiplex bead-based assay for detection of hepatitis C virus 10.1128/CVI.00265-10

In silico analysis of amino acid variation in human respiratory syncytial virus: Insights into immunodiagnostics 10.1590/0074-02760170013

Trypanosoma cruzi heparin-binding proteins and the nature of the host cell heparan sulfate-binding domain 10.1016/j.micpath.2007.10.003

Performance assessment of four chimeric Trypanosoma cruzi antigens based on antigen-antibody detection for diagnosis of chronic chagas disease 10.1371/journal.pone.0161100