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Proteomic Identification of M.tuberculosis Protein Kinase Substrates: PknB Recruits GarA, a FHA Domain-containing Protein, Through Activation Loop-mediated Interactions

Villarino, A. Duran, R. Wehenkel, A. Fernandez, P. England, P. Brodin, P. Cole, S. T. Zimny-Arndt, U. Jungblut, P. R. Cervenansky, C. Alzari, P. M.

Journal of Molecular Biology, 2005

https://doi.org/10.1016/j.jmb.2005.05.049

Priscille Brodin

Brodin Priscille

ORCID: 0000-0003-0991-7344

Email: priscille.brodin@inserm.fr

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Rosario Durán

Durán Rosario

ORCID: 0000-0002-8575-9651

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Pedro Alzari

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Structural Basis for Feed-Forward Transcriptional Regulation of Membrane Lipid Homeostasis in Staphylococcus aureus 10.1371/journal.ppat.1003108

Substrate-selective inhibition of protein kinase PDK1 by small compounds that bind to the PIF-pocket allosteric docking site 10.1016/j.chembiol.2012.07.017

Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria. 10.1074/jbc.m112.368191

Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis 10.1016/j.febslet.2012.04.034

Allosteric regulation of protein kinase PKCζ by the N-terminal C1 domain and small compounds to the PIF-pocket 10.1016/j.chembiol.2011.08.010

Functional plasticity and allosteric regulation of α-ketoglutarate decarboxylase in central mycobacterial metabolism 10.1016/j.chembiol.2011.06.004

Three-dimensional structure of rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily. 10.1073/pnas.91.7.2517

Structural implications of somatic mutations during the immune response to 2-phenyloxazolone. 10.1016/s0923-2494(94)80041-3

Crystallization and preliminary diffraction analysis of the catalytic domain of xylanase Z from Clostridium thermocellum 10.1006/jmbi.1994.1089

Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex. 10.1073/pnas.90.16.7711

Crystallization and preliminary crystallographic analysis of a tetrameric isolectin from Vicia villosa, specific for the Tn antigen 10.1006/jmbi.1993.1180

Crystallization, preliminary X‐ray diffraction study, and crystal packing of a complex between anti‐Hen lysozyme antibody F9.13.7 and Guinea‐fowl lysozyme 10.1002/prot.340150211

Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody 10.1073/pnas.89.20.9429

The three-dimensional structure of the aspartyl protease from the HIV-1 isolate BRU 10.1016/0300-9084(91)90169-2

Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein. 10.1093/nar/19.19.5237

Crystallization and preliminary X-ray diffraction data of the Fab fragment of a monoclonal antibody against apamin, a bee venom neurotoxin. 10.1016/0014-5793(91)80941-u

Resolution of hypervariable regions in T-cell receptor β chains by a modified Wu-Kabat index of amino acid diversity 10.1073/pnas.87.23.9138

Poly(Glu60Ala30Tyr10) (GAT)-induced IgG monoclonal antibodies cross-react with various self and non-self antigens through the complementarity determining regions. Comparison with IgM monoclonal polyreactive natural antibodies. 10.1002/eji.1830201104

The protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicase 10.1016/0092-8674(89)90125-6

Three-dimensional structure of Fab R19.9, a monoclonal murine antibody specific for the p-azobenzenearsonate group 10.1073/pnas.86.2.607

Three-dimensional structure of antibodies. 10.1146/annurev.iy.06.040188.003011

Preliminary crystallographic study of a complex between an heteroclitic anti-hen egg-white lysozyme antibody and the heterologous antigen pheasant egg-white lysozyme. 10.1016/0022-2836(87)90132-x

Preliminary crystallographic study of the Fab fragment of a monoclonal antibody directed against a cobra cardiotoxin 10.1016/0022-2836(86)90503-6

Crystallization and preliminary X-ray diffraction study of an endoglucanase from Clostridium thermocellum 10.1016/0022-2836(86)90396-7

3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation. 10.1074/jbc.m111.253260

Crystal structure of an enzymatically inactive trans-sialidase-like lectin from Trypanosoma cruzi: The carbohydrate binding mechanism involves residual sialidase activity 10.1016/j.bbapap.2011.04.012

Biological and structural characterization of the Mycobacterium smegmatis nitroreductase NfnB, and its role in benzothiazinone resistance 10.1111/j.1365-2958.2010.07277.x

Molecular basis of phosphatidyl-myo-inositol mannoside biosynthesis and regulation in mycobacteria. 10.1074/jbc.r110.168328

A novel role of malonyl-ACP in lipid homeostasis 10.1021/bi100136n

Crystal structure of Mycobacterium tuberculosis LppA, a lipoprotein confined to pathogenic mycobacteria 10.1002/prot.22603

Structural plasticity and distinct drug-binding modes of LfrR, a mycobacterial efflux pump regulator. 10.1128/jb.00631-09

Structure of Mycobacterium tuberculosis Rv2714, a representative of a duplicated gene family in Actinobacteria. 10.1107/s1744309109035027

Structural plasticity and catalysis regulation of a thermosensor histidine kinase 10.1073/pnas.0906699106

Structure and allosteric effects of low-molecular-weight activators on the protein kinase PDK1 10.1038/nchembio.208

Mechanisms determining cell membrane expression of different γδ TCR chain pairings 10.1002/eji.200939345

Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis. 10.1074/jbc.m109.003947

Bacterial Metabolism under FHA Control 10.1016/j.str.2009.03.004

Functional in vitro assembly of the integral membrane bacterial thermosensor DesK 10.1016/j.pep.2009.02.006

Genome-wide regulon and crystal structure of BlaI (Rv1846c) from Mycobacterium tuberculosis 10.1111/j.1365-2958.2008.06583.x

The FHA-containing protein GarA acts as a phosphorylation-dependent molecular switch in mycobacterial signaling 10.1016/j.febslet.2008.12.036

Discovery of novel inhibitors of Trypanosoma cruzi trans-sialidase from in silico screening 10.1016/j.bmcl.2008.12.065

Regulation of glutamate metabolism by protein kinases in mycobacteria 10.1111/j.1365-2958.2008.06489.x

Structural insights into sialic acid enzymology 10.1016/j.cbpa.2008.06.017

Artificial Binding Proteins (Affitins) as Probes for Conformational Changes in Secretin PulD 10.1016/j.jmb.2008.09.016

Rising standards for tuberculosis drug development 10.1016/j.tips.2008.08.001

Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis 10.1021/bi7024832

Crystal structure of a monoclonal antibody directed against an antigenic determinant common to Ogawa and Inaba serotypes of Vibrio cholerae O1 10.1002/prot.21609

Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD 10.1073/pnas.0702963104

Towards new tuberculosis drugs. 10.1042/bst0351321

Structural and Binding Studies of the Three-metal Center in Two Mycobacterial PPM Ser/Thr Protein Phosphatases 10.1016/j.jmb.2007.09.076

The crystal structure of Trypanosoma cruzi arginine kinase 10.1002/prot.21557

Mycobacterial Ser/Thr protein kinases and phosphatases: Physiological roles and therapeutic potential 10.1016/j.bbapap.2007.08.006

The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria 10.1110/ps.072982707

Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. 10.1074/jbc.m702676200

Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives - A screening based on affinity for the UMP-CMP binding site 10.1111/j.1742-4658.2007.05902.x

Insights into the Catalytic Mechanism of PPM Ser/Thr Phosphatases from the Atomic Resolution Structures of a Mycobacterial Enzyme 10.1016/j.str.2007.06.002

Molecular recognition and interfacial catalysis by the essential phosphatidylinositol mannosyltransferase PimA from mycobacteria. 10.1074/jbc.m702087200

Insights into the inter-ring plasticity of caseinolytic proteases from the X-ray structure of Mycobacterium tuberculosis ClpP1. 10.1107/s0907444906050530

Benzoic acid and pyridine derivatives as inhibitors of Trypanosoma cruzi trans-sialidase 10.1016/j.bmc.2006.12.024

The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria. 10.1128/jb.01435-06

Allosteric activation of the protein kinase PDK1 with low molecular weight compounds 10.1038/sj.emboj.7601416

Structure of armadillo ACBP: a new member of the acyl-CoA-binding protein family. 10.1107/s1744309106038164

Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. 10.1107/s0907444906030915

SPINE bioinformatics and data-management aspects of high-throughput structural biology. 10.1107/s090744490602991x

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE. 10.1107/s0907444906029775

The Ser/Thr protein kinase PknB is essential for sustaining mycobacterial growth. 10.1128/jb.00963-06

Structural basis of lipid biosynthesis regulation in Gram-positive bacteria 10.1038/sj.emboj.7601284

Peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons 10.1016/j.freeradbiomed.2006.08.010

Continuous fluorimetric assay for high-throughput screening of inhibitors of trans-sialidase from Trypanosoma cruzi 10.1016/j.ab.2006.07.018

The crystal structure of Mycobacterium tuberculosis adenylate kinase in complex with two molecules of ADP and Mg2+ supports an associative mechanism for phosphoryl transfer. 10.1110/ps.062163406

The structure of PknB in complex with mitoxantrone, an ATP-competitive inhibitor, suggests a mode of protein kinase regulation in mycobacteria 10.1016/j.febslet.2006.04.046

Crystal structure, catalytic mechanism, and mitogenic properties of Trypanosoma cruzi proline racemase 10.1073/pnas.0509010103

Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase. 10.1074/jbc.m510677200

Identification of the critical residues involved in peptidoglycan detection by Nod1. 10.1074/jbc.m509537200

Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases 10.1016/j.bbrc.2005.05.173

Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions 10.1016/j.jmb.2005.05.049

Structure and mechanism of the alkyl hydroperoxidase AhpC, a key element of the Mycobacterium tuberculosis defense system against oxidative stress. 10.1074/jbc.m503076200

Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis. 10.1107/s1744309105012364

TB - A new target, a new drug 10.1126/science.1108379

A sialidase mutant displaying trans-sialidase activity 10.1016/j.jmb.2004.09.031

First structural glimpse at a bacterial Ser/Thr protein phosphatase 10.1016/j.str.2004.10.003

Crystal structure of glycogen synthase: Homologous enzymes catalyze glycogen synthesis and degradation 10.1038/sj.emboj.7600324

Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase 10.1016/j.str.2004.02.036

Sub-families of α/β Barrel Enzymes: A New Adenine Deaminase Family 10.1016/j.jmb.2003.10.005

Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: Tyrosine is the catalytic nucleophile 10.1021/ja0344967

Preliminary crystallographic studies of glycogen synthase from Agrobacterium tumefaciens. 10.1107/s0907444902023119

The crystal structure of a plant lectin in complex with the Tn antigen. 10.1016/s0014-5793(03)00037-1

Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis. 10.1074/jbc.m300660200

The high resolution structures of free and inhibitor-bound Trypanosoma rangeli sialidase and its comparison with T. cruzi trans-sialidase. 10.1016/s0022-2836(02)01306-2

The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis. 10.1016/s1097-2765(02)00680-9

Physical estimation of triplet phases from two new proteins. 10.1107/s0907444902012222

Advantages of high-resolution phasing: MAD to atomic resolution. 10.1107/s0907444902011368

The crystal structure and catalytic mechanism of cellobiohydrolase CelS, the major enzymatic component of the Clostridium thermocellum Cellulosome. 10.1016/s0022-2836(02)00497-7

The trans-sialidase from the African trypanosome Trypanosoma brucei 10.1046/j.1432-1033.2002.02968.x

The crystal structure of the mouse apoptosis-inducing factor aif 10.1038/nsb793

1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module. 10.1023/a:1015372902151

Crystallization and preliminary X-ray analysis of the hydroperoxidase I C-terminal domain from Escherichia coli. 10.1107/s0907444902004201

Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis. 10.1107/s0907444902002676

Induction of protective immunity by synthetic Vibrio cholerae hexasaccharide derived from V. cholerae O1 Ogawa lipopolysaccharide bound to a protein carrier 10.1086/339583

Atomic (0.94 Å) resolution structure of an inverting glycosidase in complex with substrate 10.1006/jmbi.2001.5404

Mapping by site-directed mutagenesis of the region responsible for cohesin-dockerin interaction on the surface of the seventh cohesin domain of Clostridium thermocellum CipA. 10.1021/bi011854e

Duplicated dockerin subdomains of Clostridium thermocellum endoglucanase Ce1D bind to a cohesin domain of the scaffolding protein CipA with distinct thermodynamic parameters and a negative cooperativity 10.1021/bi011853m

Probing molecular function of trypanosomal sialidases: Single point mutations can change substrate specificity and increase hydrolytic activity 10.1093/glycob/11.4.305

Epitope mapping of trans-sialidase from Trypanosoma cruzi reveals the presence of several cross-reactive determinants. 10.1128/iai.69.3.1869-1875.2001

Can isotype switch modulate antigen-binding affinity and influence clonal selection? 10.1002/1521-4141(2000012)30:12<3387::aid-immu3387>3.0.co;2-k

Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae 01 in complex with antigen: Molecular basis for serotype specificity 10.1073/pnas.060022997

Crystal structure of the allergen Equ c 1. A dimeric lipocalin with restricted IgE-reactive epitopes. 10.1074/jbc.m002854200

Production and functional characterization of two mouse/human chimeric antibodies with specificity for the tumor-associated Tn-antigen 10.1089/02724570050109620

Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface plasmon resonance spectroscopy. 10.1016/s0014-5793(00)01248-5

Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds 10.1046/j.1432-1327.2000.01141.x

The first α helix of interleukin (IL)-2 folds as a homotetramer, acts as an agonist of the IL-2 receptor β chain, and induces lymphokine-activated killer cells 10.1084/jem.191.3.529

Structural basis of sialyltransferase activity in trypanosomal sialidases. 10.1093/emboj/19.1.16

Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase 10.1073/pnas.96.11.6445

Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginine. 10.1110/ps.8.4.934

Crystallization and preliminary crystallographic analysis of the major horse allergen Equ c 1. 10.1107/s0907444998015510

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis 10.1042/0264-6021:3380753

Structure of catalase-A from Saccharomyces cerevisiae 10.1006/jmbi.1998.2453

Mitochondrial release of caspase-2 and -9 during the apoptotic process 10.1084/jem.189.2.381

Structural features involved in the formation of a complex between the monomeric or the dimeric form of the Rev-erbβ DNA-binding domain and its DNA reactive sites 10.1021/bi980748i

The cellulosome of Clostridium thermocellum. 10.1042/bst0260178

Phage-displayed mimotopes elicit monoclonal antibodies specific for a malaria vaccine candidate. 10.1515/bchm.1998.379.1.65

The carboxy-terminal C2-like domain of the alpha-toxin from Clostridium perfringens mediates calcium-dependent membrane recognition. 10.1046/j.1365-2958.1997.6161993.x

The crystal structure of a type I cohesin domain at 1.7 Å resolution 10.1006/jmbi.1997.1326

Molecular cloning of a monoclonal anti-tumor antibody specific for the Tn antigen and expression of an active single-chain Fv fragment 10.1089/hyb.1997.16.317

The MBP fusion protein restores the activity of the first phosphatase domain of CD45. 10.1016/s0014-5793(97)00703-5

Amino acid sequence and three-dimensional structure of the Tn-specific isolectin B4 from Vicia villosa. 10.1016/s0014-5793(97)00677-7

Analysis of human IL-2/IL-2 receptor β chain interactions: Monoclonal antibody H2-8 and new IL-2 mutants define the critical role of α helix-A of IL-2 10.1006/cyto.1996.0192

Crystallization and preliminary structural analysis of catalase A from Saccharomyces cerevisiae. 10.1002/pro.5560060229

cDNA cloning and sequencing reveal the major horse allergen Equ c1 to be a glycoprotein member of the lipocalin superfamily 10.1074/jbc.271.51.32951

Interleukin 2 and its receptors: recent advances and new immunological functions. 10.1016/0167-5699(96)10057-c

Subcloning of a DNA fragment encoding a single cohesin domain of the Clostridium thermocellum cellulosome-integrating protein CipA: purification, crystallization, and preliminary diffraction analysis of the encoded polypeptide. 10.1002/pro.5560050623

Crystallization of a family 8 cellulase from Clostridium thermocellum. 10.1002/(sici)1097-0134(199605)25:1<134::aid-prot12>3.3.co;2-j

The crystal structure of a family 5 endoglucanase mutant in complexed and uncomplexed forms reveals an induced fit activation mechanism 10.1006/jmbi.1996.0222

The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum. 10.1016/s0969-2126(96)00031-7

A potent new mode of beta-lactamase inhibition revealed by the 1.7 A X-ray crystallographic structure of the TEM-1-BLIP complex. 10.1038/nsb0396-290

Conservative substitutions in the hydrophobic core of Rhodobacter sphaeroides thioredoxin produce distinct functional effects 10.1002/pro.5560041207

A common protein fold and similar active site in two distinct families of β-glycanases 10.1038/nsb0795-569

Crystal structure of a cross-reaction complex between Fab F9.13.7 and Guinea fowl lysozyme 10.1074/jbc.270.30.18067

Multiple crystal forms of endoglucanase CelD: signal peptide residues modulate lattice formation. 10.1016/s0022-2836(95)80045-x

Structural and functional analysis of the metal-binding sites of Clostridium thermocellum endoglucanase CelD 10.1074/jbc.270.17.9757

Missense mutations in the catalase‐peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis 10.1111/j.1365-2958.1995.tb02238.x

The human pre-B cell receptor: Structural constraints for a tentative model of the pseudo-light (ΨL) chain 10.1016/0161-5890(94)90105-8

Characterization of two crystal forms of Clostridium thermocellum endoglucanase CelC 10.1002/prot.340190209

Crystal structures of pheasant and guinea fowl egg‐white lysozymes 10.1002/pro.5560030508

Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase 10.1016/j.str.2017.04.011

PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis 10.1371/journal.ppat.1006399

The crystal structure of PknI from Mycobacterium tuberculosis shows an inactive, pseudokinase-like conformation 10.1111/febs.14003

Can isotype switch modulate antigen-binding affinity and influence clonal selection? 10.1002/1521-4141(2000012)30:12<3387::AID-IMMU3387>3.0.CO;2-K

Erratum: Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens (Acta Crystallographica Section D: Biological Crystallography (2006) D62 (1196-1207)) 10.1107/S0907444906044246

Neutron diffraction analysis of barium nitroprusside trihydrate at room temperature 10.1016/0022-4596(90)90290-E

Studies of structure and specificity of some antigen-antibody complexes. 10.1098/rstb.1989.0026

Structure of pivaloyloxymethyl (3S,5R)‐penicillanate 1,1‐dioxide 10.1107/S0108270188003609

Molecular structure and semiexternal molecular motions in 2,4,5-trichlorobenzenesulfonyl chloride, C6H2Cl4O2S 10.1007/BF01161141

Three-dimensional structure and antigen binding specificity of antibodies 10.1016/0300-9084(90)90114-V

Crystallization of a family 8 cellulase from Clostridium thermocellum 10.1002/(SICI)1097-0134(199605)25:1<134::AID-PROT12>3.0.CO;2-L

Molecular recognition of artificial single-electron acceptor cosubstrates by glucose oxidase? 10.1021/ja960197o

Molecular replacement and high-throughput structure determination 10.1524/zkri.217.12.715.20653

Low‐resolution phase extension and refinement by maximum entropy 10.1107/S0108767388002971

Characterization of a UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase with an unusual lectin domain from the platyhelminth parasite Echinococcus granulosus 10.1042/BJ20031877

Structure-based virtual screening to get new scaffold inhibitors of the Ser/Thr protein kinase PknB from mycobacterium tuberculosis 10.2174/1570180813666160801162204

The reaction mechanism of metallo-lactamases is tuned by the conformation of an active-site mobile loop 10.1128/AAC.01754-18

Structural insights into the functional versatility of an FHA domain protein in mycobacterial signaling 10.1126/scisignal.aav9504

Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance 10.1016/j.jsb.2018.08.001

Novel mechanistic insights into physiological signaling pathways mediated by mycobacterial Ser/Thr protein kinases 10.1038/s41435-019-0069-9

The crystal structure of Rv2991 from Mycobacterium tuberculosis: An F 420 binding protein with unknown function 10.1016/j.jsb.2019.03.006

New substrates and interactors of the mycobacterial Serine/Threonine protein kinase PknG identified by a tailored interactomic approach 10.1016/j.jprot.2018.09.013

The synthesis and kinetic evaluation of aryl α-aminophosphonates as novel inhibitors of T. cruzi trans-sialidase 10.1016/j.ejmech.2018.08.089

Double autoinhibition mechanism of signal transduction ATPases with numerous domains (STAND) with a tetratricopeptide repeat sensor 10.1093/nar/gkz112

Overall Structures of Mycobacterium tuberculosis DNA Gyrase Reveal the Role of a Corynebacteriales GyrB-Specific Insert in ATPase Activity 10.1016/j.str.2019.01.004

High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur 10.3390/molecules24244451

Patrick ENGLAND

ENGLAND Patrick

ORCID: 0000-0001-6410-5918

Email: patrick.england@pasteur.fr

A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca2+ and Mg2+ binding to EDTA 10.1007/s00249-021-01523-7

SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system 10.1038/s41467-021-23099-8

Protein intrinsic viscosity determination with the Viscosizer TD instrument: reaching beyond the initially expected applications 10.1007/s00249-020-01492-3

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum 10.1038/s41467-020-15490-8

Interaction of protocadherin-15 with the scaffold protein whirlin supports its anchoring of hair-bundle lateral links in cochlear hair cells 10.1038/s41598-020-73158-1

High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics 10.1038/s41587-020-0466-7

Structural basis for the increased processivity of D- family DNA polymerases in complex with PCNA 10.1038/s41467-020-15392-9

Structure of the prefusion-locking broadly neutralizing antibody RVC20 bound to the rabies virus glycoprotein 10.1038/s41467-020-14398-7

X-ray structures of the post-fusion 6-helix bundle of the human syncytins and their functional implications 10.1016/j.jmb.2019.10.020

An extracellular Leptospira interrogans leucine‐rich repeat protein binds human E‐ and VE‐cadherins 10.1111/cmi.12949

Calcium-dependent disorder-to-order transitions are central to the secretion and folding of the CyaA toxin of Bordetella pertussis, the causative agent of whooping cough 10.1016/j.toxicon.2018.01.007

Quality assessment and optimization of purified protein samples: why and how? 10.1186/s12934-014-0180-6

Clostridium difficile toxin expression is inhibited by the novel regulator TcdC 10.1111/j.1365-2958.2007.05739.x

Single-domain antibodies recognize selectively small oligomeric forms of amyloid beta, prevent Abeta-induced neurotoxicity and inhibit fibril formation. 10.1016/j.molimm.2008.09.008

The Stable Interaction Between Signal Peptidase LepB of Escherichia coli and Nuclease Bacteriocins Promotes Toxin Entry into the Cytoplasm. 10.1074/jbc.M115.691907

Specificity and affinity of human Fc{gamma} receptors and their polymorphic variants for human IgG subclasses. 10.1182/blood-2008-09-179754

Insights into the Rrf2 repressor family - the structure of CymR, the global cysteine regulator of Bacillus subtilis 10.1111/j.1742-4658.2011.08195.x

Crl Binds to Domain 2 of S and Confers a Competitive Advantage on a Natural rpoS Mutant of Salmonella enterica Serovar Typhi 10.1128/JB.00801-10

A short synthetic peptide inhibits signal transduction, migration and angiogenesis mediated by Tie2 receptor. 10.1038/sj.embor.7400100

Structural Characterization of the Stem–Stem Dimerization Interface between Prolactin Receptor Chains Complexed with the Natural Hormone 10.1016/j.jmb.2010.09.036

FcgammaRIV is a mouse IgE receptor that resembles macrophage FcepsilonRI in humans and promotes IgE-induced lung inflammation. 10.1172/JCI36452

Peroxynitrite transforms nerve growth factor into an apoptotic factor for motor neurons 10.1016/j.freeradbiomed.2006.08.010

Phosphorylcholine-carbohydrate-protein conjugates efficiently induce hapten-specific antibodies which recognize both Streptococcus pneumoniae and Neisseria meningitidis: a potential multitarget vaccine against respiratory infections. 10.1021/jm040783p

Proteomic Identification of M.tuberculosis Protein Kinase Substrates: PknB Recruits GarA, a FHA Domain-containing Protein, Through Activation Loop-mediated Interactions 10.1016/j.jmb.2005.05.049

Analysis of the Escherichia coli glucosamine-6-phosphate synthase activity by isothermal titration calorimetry and differential scanning calorimetry 10.1016/j.abb.2010.04.010

Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR. 10.1371/journal.pone.0089502

Facile immobilization of DNA using an enzymatic his-tag mimic 10.1039/c7cc07207d

Novel svVEGF isoforms from Macrovipera lebetina venom interact with neuropilins. 10.1016/j.bbrc.2009.08.068

The high-affinity human IgG receptor FcγRI (CD64) promotes IgG-mediated inflammation, anaphylaxis, and antitumor immunotherapy. 10.1182/blood-2012-07-442541

Crystal Structure of an Affinity-matured Prolactin Complexed to Its Dimerized Receptor Reveals the Topology of Hormone Binding Site 2 10.1074/jbc.M109.089128

Mycobacterial Ser/Thr protein kinases and phosphatases: physiological roles and therapeutic potential. 10.1016/j.bbapap.2007.08.006

Structural and Thermodynamic Bases for the Design of Pure Prolactin Receptor Antagonists 10.1074/jbc.M704364200

The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4. 10.1038/emboj.2009.209

Structural Basis for the ABO Blood-Group Dependence of Plasmodium falciparum Rosetting 10.1371/journal.ppat.1002781

Correction: The Translocation Domain of Botulinum Neurotoxin A Moderates the Propensity of the Catalytic Domain to Interact with Membranes at Acidic pH. 10.1371/journal.pone.0161743

The Translocation Domain of Botulinum Neurotoxin A Moderates the Propensity of the Catalytic Domain to Interact with Membranes at Acidic pH 10.1371/journal.pone.0153401

Rabies virus matrix protein interplay with eIF3, new insights into rabies virus pathogenesis. 10.1093/nar/gkl1127

Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus. 10.1111/j.1742-4658.2005.05045.x

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin. 10.1016/j.bpj.2010.10.016

A Residue Quartet in the Extracellular Domain of the Prolactin Receptor Selectively Controls Mitogen-activated Protein Kinase Signaling 10.1074/jbc.M115.639096

The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation 10.3390/toxins8040090

Structural and functional features of Crl proteins and identification of conserved surface residues required for interaction with the RpoS/σS subunit of RNA polymerase. 10.1042/BJ20140578

The catalytic domains of C lostridium sordellii lethal toxin and related large clostridial glucosylating toxins specifically recognize the negatively charged phospholipids phosphatidylserine and phosphatidic acid 10.1111/cmi.12449

Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery 10.1002/1873-3468.12729

Residue 146 regulates prolactin receptor folding, basal activity and ligand-responsiveness: Potential implications in breast tumorigenesis 10.1016/j.mce.2014.12.006

The stress sigma factor of RNA polymerase RpoS/σ S is a solvent-exposed open molecule in solution 10.1042/BCJ20170768

The disulfide bonds in glycoprotein e2 of hepatitis C virus reveal the tertiary organization of the molecule. 10.1371/journal.ppat.1000762

Structural basis of potent Zika–dengue virus antibody cross-neutralization 10.1038/nature18938

Attenuation of rabies virulence: takeover by the cytoplasmic domain of its envelope protein. 10.1126/scisignal.2000510

High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid 10.1038/srep41662

Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc 10.1371/journal.ppat.1005813

SUN proteins belong to a novel family of β-(1,3)-glucan-modifying enzymes involved in fungal morphogenesis. 10.1074/jbc.M112.440172

Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring 10.1038/nbt.3964

IgG subclasses determine pathways of anaphylaxis in mice 10.1016/j.jaci.2016.03.028

A Druggable Pocket at the Nucleocapsid/Phosphoprotein Interaction Site of Human Respiratory Syncytial Virus 10.1128/JVI.01612-15

Identification of an in vivo orally active dual-binding protein-protein interaction inhibitor targeting TNFα through combined in silico/in vitro/in vivo screening 10.1038/s41598-017-03427-z

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes 10.7554/eLife.17523

Structure of a Plasmodium falciparum PfEMP1 rosetting domain reveals a role for the N-terminal segment in heparin-mediated rosette inhibition 10.1073/pnas.1018692108

Transducing properties of a pre-structured α-helical DPT-peptide containing a short canine adenovirus type 2 E4orf4 PP2A1-binding sequence 10.1016/j.bbagen.2013.02.024

A glycerophospholipid-specific pocket in the RVFV class II fusion protein drives target membrane insertion 10.1126/science.aal2712